Identification of phenotypically and functionally heterogeneous mouse mucosal-associated invariant T cells using MR1 tetramers

A Rahimpour; HF Koay; A Enders; R Clanchy; SBG Eckle; B Meehan; Z Chen; B Whittle; L Liu; DP Fairlie; CC Goodnow; J McCluskey; J Rossjohn; AP Uldrich; DG Pellicci; DI Godfrey

Journal article

Identification of phenotypically and functionally heterogeneous mouse mucosal-associated invariant T cells using MR1 tetramers

A Rahimpour, HF Koay, A Enders, R Clanchy, SBG Eckle, B Meehan, Z Chen, B Whittle, L Liu, DP Fairlie, CC Goodnow, J McCluskey, J Rossjohn, AP Uldrich, DG Pellicci, DI Godfrey

Journal of Experimental Medicine | Published : 2015

DOI: 10.1084/jem.20142110

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Abstract

Studies on the biology of mucosal-associated invariant T cells (MAIT cells) in mice have been hampered by a lack of specific reagents. Using MR1-antigen (Ag) tetramers that specifically bind to the MR1-restricted MAIT T cell receptors (TCRs), we demonstrate that MAIT cells are detectable in a broad range of tissues in C57BL/6 and BALB/c mice. These cells include CD4-CD8-, CD4-CD8+, and CD4+CD8- subsets, and their frequency varies in a tissue- and strain-specific manner. Mouse MAIT cells have a CD44hiCD62Llo memory phenotype and produce high levels of IL-17A, whereas other cytokines, including IFN-γ, IL-4, IL-10, IL-13, and GM-CSF, are produced at low to moderate levels. Consistent with high ..

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University of Melbourne Researchers

Fern Koay Author

Sidonia Eckle Author

Bronwyn Meehan Author

Zhenjun Chen Author

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Grants

Awarded by National Institutes of Health

Funding Acknowledgements

This work was supported by a project grant and program grants from the National Health and Medical Research Council of Australia (NHMRC; 108394, 1013667, and 1016629) and the Australian Research Council (ARC; CE140100011 and LE110100106). A. Enders is supported by an NHMRC Career Development Fellowship (1035858); D.G. Pellicci is supported by an NHMRC Early Career Fellowship (1054431); A.P. Uldrich is supported by an ARC Future Fellowship (FT140100278); D.I. Godfrey and D.P. Fairlie are supported by NHMRC Senior Principal Research Fellowships (1020770 and 1027369); J. Rossjohn is supported by an NHMRC Australia Fellowship (AF50).